DOT Physical Exams - Cincinnati, OH

Locations, All Cities, All States

FMCSA | FAA | USCG | FTA | FRA | PHMSA

With 38 testing centers in the Cincinnati area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

Schedule Your Test

38 DOT Drug-Testing Centers in Cincinnati

Minutes Away, Test Today

375 Dixmyth Ave 7th Fl

1.03 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 04:00 PM, 01:00 PM - 04:00 PM
375 Dixmyth Ave 7th Fl
Cincinnati, OH 45220
Call (800) 221-4291 Get Donor Pass

219 W 9th St

1.56 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 11:00 AM - 02:00 PM
219 W 9th St
Cincinnati, OH 45202
Call (800) 221-4291 Get Donor Pass

1150 W 8th St Ste 120

1.95 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 10:30 AM, 12:30 PM - 01:30 PM
1150 W 8th St Ste 120
Cincinnati, OH 45203
Call (800) 221-4291 Get Donor Pass

118 W 5th St

2.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
118 W 5th St
Covington, KY 41011
Call (800) 221-4291 Get Donor Pass

4592 Montgomery Rd

3.87 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 02:00 PM
4592 Montgomery Rd
Cincinnati, OH 45212
Call (800) 221-4291 Get Donor Pass

4623 Wesley Ave Ste C

4.20 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:30 AM
4623 Wesley Ave Ste C
Cincinnati, OH 45212
Call (800) 221-4291 Get Donor Pass

3802 Paxton Ave Ste 1

4.64 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 01:00 PM - 03:30 PM
3802 Paxton Ave Ste 1
Cincinnati, OH 45209
Call (800) 221-4291 Get Donor Pass

3158 Glenmore Ave

5.40 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 12:30 PM - 02:00 PM
3158 Glenmore Ave
Cincinnati, OH 45211
Call (800) 221-4291 Get Donor Pass

5936 Glenway Ave

5.57 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
5936 Glenway Ave
Cincinnati, OH 45238
Call (800) 221-4291 Get Donor Pass

5002 Ridge Ave

5.61 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 03:30 PM
5002 Ridge Ave
Cincinnati, OH 45209
Call (800) 221-4291 Get Donor Pass

4767 N Bend Rd

5.70 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
4767 N Bend Rd
Cincinnati, OH 45211
Call (800) 221-4291 Get Donor Pass

4387 Winston Ave

5.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4387 Winston Ave
Covington, KY 41015
Call (800) 221-4291 Get Donor Pass

2446 Kipling Ave

6.01 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 12:30 PM, 01:30 PM - 04:00 PM
2446 Kipling Ave
Cincinnati, OH 45239
Call (800) 221-4291 Get Donor Pass

5920 Colerain Ave

6.27 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 12:00 PM, 01:30 PM - 04:00 PM
5920 Colerain Ave
Cincinnati, OH 45239
Call (800) 221-4291 Get Donor Pass

3328 Westbourne Dr

6.28 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM✓ Walk-in with Electronic Donor Pass
3328 Westbourne Dr
Cincinnati, OH 45248
Call (800) 221-4291 Get Donor Pass

2019 Alexandria Pike

6.75 mi
🕑 Testing Hours: M-Th 9:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
2019 Alexandria Pike
Highland Heights, KY 41076
Call (800) 221-4291 Get Donor Pass

2475 W Galbraith Rd Ste B,

7.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2475 W Galbraith Rd Ste B,
Cincinnati, OH 45239
Call (800) 221-4291 Get Donor Pass

939 Dudley Pike

7.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
939 Dudley Pike
Edgewood, KY 41017
Call (800) 221-4291 Get Donor Pass

8634 Reading Rd

7.27 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 04:00 PM
8634 Reading Rd
Cincinnati, OH 45215
Call (800) 221-4291 Get Donor Pass

3699 Alexandria Pike

7.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3699 Alexandria Pike
Cold Spring, KY 41076
Call (800) 221-4291 Get Donor Pass

1825 Airport Exchange Blvd Ste 100

7.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1825 Airport Exchange Blvd Ste 100
Erlanger, KY 41018
Call (800) 221-4291 Get Donor Pass

8459 Colerain Ave

7.83 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:00 AM, 01:00 PM - 04:00 PM
8459 Colerain Ave
Cincinnati, OH 45239
Call (800) 221-4291 Get Donor Pass

2616 Legends Way Ste B,

7.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2616 Legends Way Ste B,
Crestview Hills, KY 41017
Call (800) 221-4291 Get Donor Pass

500 Thomas More Pkwy #8

7.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 Thomas More Pkwy #8
Crestview Hills, KY 41017
Call (800) 221-4291 Get Donor Pass

200 Medical Village Dr

8.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Medical Village Dr
Edgewood, KY 41017
Call (800) 221-4291 Get Donor Pass

650 Sprucewood Ln

8.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
650 Sprucewood Ln
Erlanger, KY 41018
Call (800) 221-4291 Get Donor Pass

3413 Dixie Hwy

8.86 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3413 Dixie Hwy
Erlanger, KY 41018
Call (800) 221-4291 Get Donor Pass

Donaldson Hwy

8.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Donaldson Hwy
Erlanger, KY 41018
Call (800) 221-4291 Get Donor Pass

G Center N Ky Airport

9.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
G Center N Ky Airport
Erlanger, KY 41018
Call (800) 221-4291 Get Donor Pass

7235 Beechmont Ave,

9.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7235 Beechmont Ave,
Cincinnati, OH 45230
Call (800) 221-4291 Get Donor Pass

Five Mile Rd

9.34 mi
🕑 Facility: Mon–Fri: 08:30 AM - 04:30 PM (Lunch: 12:30 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:30 AM, 01:00 PM - 03:30 PM
Five Mile Rd
Cincinnati, OH 45255
Call (800) 221-4291 Get Donor Pass

10575 Springfield Pike

9.60 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 01:00 PM - 04:00 PM
10575 Springfield Pike
Cincinnati, OH 45215
Call (800) 221-4291 Get Donor Pass

7370 Turfway Rd Ste 170,

9.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7370 Turfway Rd Ste 170,
Florence, KY 41042
Call (800) 221-4291 Get Donor Pass

10475 Reading Rd,

10.12 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM (Lunch: 12:30 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 01:00 PM, 02:00 PM - 04:30 PM
10475 Reading Rd,
Cincinnati, OH 45241
Call (800) 221-4291 Get Donor Pass

Po Box 718

10.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 718
Hebron, KY 41048
Call (800) 221-4291 Get Donor Pass

9030 Montgomery Rd

10.59 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
9030 Montgomery Rd
Cincinnati, OH 45242
Call (800) 221-4291 Get Donor Pass

9275 Montgomery Rd, Ste 150

10.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9275 Montgomery Rd, Ste 150
Cincinnati, OH 45242
Call (800) 221-4291 Get Donor Pass

2091 N Bend Rd

10.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2091 N Bend Rd
Hebron, KY 41048
Call (800) 221-4291 Get Donor Pass

Cincinnati, OH DOT Physical - What You Need to Know

A DOT physical is an all-encompassing health evaluation mandated by the Department of Transportation for commercial motor vehicle operators. This assessment guarantees drivers are fit on physical, mental, and emotional levels to safely handle large vehicles on public roadways. A certified medical examiner from the National Registry performs the exam. Successful completion awards a DOT medical certificate, necessary for a valid CDL per FMCSA rules. Questions? Call us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of medical history and medication assessment
  • Eye test (corrective eyewear permissible; minimum norms apply)
  • Hearing test (forced whisper test or audiometric assessment)
  • Check blood pressure and pulse
  • Physical exam (system review)
  • Urinalysis (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be valid for 24 months. In Cincinnati, MT, some conditions may demand shorter certification or specialist papers.

Who needs a DOT Physical?

To possess a valid DOT medical card, you must:

  • Hold a commercial driver's license (CDL)- class A,B, or C for interstate travel
  • Operate vehicles over 10,001 lbs. in interstate commerce
  • Transport either more than 8 hired passengers or over 15 non-hired passengers
  • Carry hazardous materials necessitating DOT-required placarding
  • Be employed by organizations under DOT regulatory entities, such as FMCSA, FAA, FRA, FTA, or USCG, where DOT medical qualification is mandated in Cincinnati, MI

DOT Physical Near Cincinnati, OH

Monfort Heights South, OH

Delhi Hills, OH

Cheviot, OH

Monfort Heights East, OH

Golf Manor, OH

Delshire, OH

Elmwood Place, OH

Norwood, OH

St. Bernard, OH

Saint Bernard, OH

Clifton, OH

Jerusalem, OH

Hamilton, OH

What to bring with you to a DOT Physical?

  • Government-Issued Picture ID
  • Glasses/Contacts & Prescription
  • Complete Medication List & Dosages
  • CPAP Data (if used)
  • Specialist Clearances (Cardiology, Sleep Apnea, Diabetes)
  • Recent A1C/Blood Pressure Logs (if monitored)
In Cincinnati, MT, ensure these documents are ready for your consultation.

What happens if I fail a DOT Physical?

  • No issuance of Medical card
  • Disqualified temporarily
  • Doctor's referral for follow-up
  • FMCSA offers exemptions for specific health conditions
  • Employer and relevant parties notified
  • Steps to address health concerns, retake the exam and meet Cincinnati, MD requirements

Understanding these elements will guide your actions following any disqualification or requirement for further medical evaluations.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Failure to see 20/40 in both eyes (even with lenses), challenges with traffic signal recognition, or inability to hear a forced whisper at 5 feet.
  • Blood Pressure & Heart Disease: Unmanaged blood pressure (≥180/110 mmHg), recent cardiac events, unmanaged angina, or an implanted defibrillator without approval.
  • Diabetes: Poorly controlled diabetes with frequent hypoglycemic events or complications impacting driving safety.
  • Sleep Apnea & Lung Problems: Unaddressed sleep apnea causing fatigue or severe respiratory issues hindering oxygen intake.
  • Neurological Disorders: Epilepsy (without exemption), conditions causing unanticipated consciousness loss, vertigo, or tremors.
  • Substance Abuse: Active illegal drug use, dependency on alcohol, or misuse of prescriptions affecting driving ability.
  • Psychiatric & Cognitive Disorders: Uncontrolled psychiatric conditions (e.g., schizophrenia or recent bipolar episodes) or cognitive issues impairing decision-making and response.

Various conditions might only temporarily disqualify you until obtaining medical documentation or proof, such as controlled hypertension. However, certain cases like untreated epilepsy or implanted defibrillators are viewed as permanent disqualifications by FMCSA standards.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

Cincinnati Drug Testing

Quickly find trusted local drug testing centers in Cincinnati, OH — fast, convenient, and reliable every time!

Cincinnati DOT Drug Testing

Quickly find a local DOT drug testing center in Cincinnati, OH — fast, reliable, convenient nationwide service!

Cincinnati DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Cincinnati, OH.

Cincinnati Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Cincinnati, OH.v

Cincinnati Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Cincinnati, OH locations—results you can trust, every time!

Cincinnati Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Cincinnati, OH.

Cincinnati Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Cincinnati, OH.

Cincinnati 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Cincinnati, OH employers and individuals nationwide.

Cincinnati 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Cincinnati, OH workplace and personal testing needs.

Cincinnati On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Cincinnati, OH workplace compliant.

Cincinnati DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Cincinnati, OH drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291