Drug & Alcohol Testing Near Me - Middleburg, FL

Accredited Drug Testing provides drug and alcohol testing services at multiple locations throughout your local area.  

Employment, DOT, Court Ordered, Personal
Urine | Hair Follicle | Oral Fluid | Blood | ETG

36 Drug-Testing Centers in Middleburg

Minutes Away, Test Today

1708 Blanding Blvd3.14 miles

1708 Blanding Blvd
Middleburg, FL 32068

Onsite Only9.17 miles

Onsite Only
Fleming Island, FL 32003

1680 Eagle Harbor Parkway, Suite B9.66 miles

1680 Eagle Harbor Parkway, Suite B
Orange Park, FL 32003

2140 Kingsley Ave9.67 miles

2140 Kingsley Ave
Orange Park, FL 32073

2020 Kingsley Ave Ste A9.69 miles

2020 Kingsley Ave Ste A
Orange Park, FL 32073

1895 Kingsley Ave Ste 8039.71 miles

1895 Kingsley Ave Ste 803
Orange Park, FL 32073

1563 Kingsley Ave Ste 10210.30 miles

1563 Kingsley Ave Ste 102
Orange Park, FL 32073

1465 Kingsley Ave Ste 1401,10.48 miles

1465 Kingsley Ave Ste 1401,
Orange Park, FL 32073

3185 Highway 1711.04 miles

3185 Highway 17
Green Cove Springs, FL 32043

705 Ferris Street11.95 miles

705 Ferris Street
Green Cove Springs, FL 32043

12303 San Jose Blvd14.65 miles

12303 San Jose Blvd
Jacksonville, FL 32223

5501 Roosevelt Blvd15.72 miles

5501 Roosevelt Blvd
Jacksonville, FL 32244

7963 Normandy Blvd16.20 miles

7963 Normandy Blvd
Jacksonville, FL 32221

6671 Hyde Grove Ave16.20 miles

6671 Hyde Grove Ave
Jacksonville, FL 32210

1584 Normandy Village Parkway Ste 3216.29 miles

1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

4616 San Juan Ave16.82 miles

4616 San Juan Ave
Jacksonville, FL 32210

1912 Hamilton St Ste 10316.97 miles

1912 Hamilton St Ste 103
Jacksonville, FL 32210

9826 San Jose Blvd16.98 miles

9826 San Jose Blvd
Jacksonville, FL 32257

9857 Old Saint Augustine Rd Ste 417.23 miles

9857 Old Saint Augustine Rd Ste 4
Jacksonville, FL 32257

1610 Blanding Blvd17.30 miles

1610 Blanding Blvd
Jacksonville, FL 32210

345 W Madison St17.37 miles

345 W Madison St
Starke, FL 32091

5964 Normandy Blvd17.73 miles

5964 Normandy Blvd
Jacksonville, FL 32205

231 Airline Way18.08 miles

231 Airline Way
Jacksonville, FL 32217

4221 Baymeadows Rd Ste 618.20 miles

4221 Baymeadows Rd Ste 6
Jacksonville, FL 32217

1550 S Water St18.66 miles

1550 S Water St
Starke, FL 32091

4498 Hendricks Ave18.96 miles

4498 Hendricks Ave
Jacksonville, FL 32207

7033 Commonwealth Ave Ste 819.34 miles

7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

9143 Philips Hwy Ste 49519.51 miles

9143 Philips Hwy Ste 495
Jacksonville, FL 32256

2063 Oak St19.90 miles

2063 Oak St
Jacksonville, FL 32204

837 Edgewood Ave N19.91 miles

837 Edgewood Ave N
Jacksonville, FL 32254

7933 Baymeadows Way Ste 320.04 miles

7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

4933-1 University Blvd W20.12 miles

4933-1 University Blvd W
Jacksonville, FL 32216

4237 Salisbury Rd Ste 30420.33 miles

4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

6639 Southpoint Pkwy Ste 10620.33 miles

6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

4063 Salisbury Rd Ste 20520.40 miles

4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

1325 San Marco Blvd Ste 30120.65 miles

1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

Drug Testing Near Me

Searching for Nearby Drug Testing Facilities?

In Middleburg, FL and across the nation, Accredited Drug Testing, Inc. stands out as a premier provider of immediate drug and alcohol testing services, offering convenience through a vast network of over 20,000 locations nationwide. Whether your requirement is for a DOT-regulated examination, a court-mandated screening, pre-employment testing, or individual assessments, we ensure the process is swift, straightforward, and confidential. Our accredited collection sites and laboratories guarantee precise, dependable results that adhere to Federal and Middleburg, FL state compliance laws. Simply dial (800) 221-4291 or book online anytime to discover a drug testing center near you.

When utmost safety, adherence to regulations, and peace of mind are paramount, Accredited Drug Testing provides reliable, punctual results every time, nationwide and in Middleburg, FL.

Drug and Alcohol Testing Options

Urine Drug Testing Available Panels
5 Panel Drug Test (DOT/NON DOT)
5 Panel + Alcohol Drug Test
5 Panel + ETG Alcohol Drug Test
10 Panel Drug Test
10 Panel + ETG Alcohol Drug Test
12 Panel Drug Test
Hair Drug Testing Available Panels
5 Panel Hair Drug Test
5 Panel + ETG Hair Drug Test
10 Panel Hair Drug Test
12 Panel Hair Drug Test
14 Panel Hair Drug Test
16 Panel Hair Drug Test
Alcohol Drug Testing Available Panels
Alcohol Urine Drug Test
ETG Alcohol Test (Urine)
Breath Alcohol Test
Blood Alcohol Test
Peth Alcohol Blood Test
ETG Alcohol Test (Hair)

Customized Testing Options Available l Discounts available for Bulk Purchases l Onsite Drug Testing Services

How Urine Drug Testing Works

Urine drug testing is the predominant method for drug screens nationwide and stands as the sole test type recognized for DOT-regulated drug testing under federal regulation 49 CFR Part 40. It successfully identifies recent substance exposure through a urine sample, searching for drugs, drug metabolites, and notable alcohol biomarkers. This method's rigorous standardization and confidentiality protocols deliver legally sound and accurate results, suitable for employers or individuals and necessary court orders in Middleburg, FL.

Testing Methods & Collection Process

1. Donor Identity Verification

To begin, the test donor presents a valid government ID and completes chain-of-custody form signatures.

2. Secure Collection Environment

The process involves collecting urine in a monitored restroom setting, eliminating tampering risks. Securing personal items, the water taps, and adding dye to toilet water are standard procedures in Middleburg, FL collections.

3. Chain of Custody Documentation

A federally approved Chain of Custody Form (CCF) is used to maintain test integrity and trace the specimen from collection onward.

4. Temperature & Validity Checks

After collection, the specimen undergoes immediate temperature and appearance checks by the collector. This is followed by lab-based validity tests, measuring specific gravity, creatinine, pH levels, and screening for adulterants.

5. DOT Split Specimen

For DOT tests, the sample is split into two vials: Bottle A for initial evaluation and Bottle B for possible retesting upon dispute. Conversely, non-DOT tests involve collecting a single vial.

6. Laboratory Screening & Confirmation

Certified laboratories, including those in Middleburg, FL, conduct testing in two phases:

  • Initial Immunoassay Screen: Identifies drug classes.
  • Confirmation Testing: Utilizes GC/MS or LC/MS technology for verifying specific drug substances and metabolites.

7. MRO Verification

A licensed Medical Review Officer (MRO) evaluates any non-negative outcomes, ensuring their veracity through review discussions with donors before final results are officially confirmed.

Duration for Receiving Urine Test Outcomes

  • Instant Urine Test: Negative results available same-day, while positives are submitted for lab reevaluation which spans 24-72 hours.
  • Lab-Based Urine Test: Returns most negative results within 24-48 hours.
  • DOT Drug Test: Typically requires 1-3 business days, contingent on MRO analysis and donor communications.
  • Urine EtG Alcohol Test: Concludes within a 24-48 hour window.

Extended timeframes might be necessitated by shy bladder conditions, invalid specimen issues, or when further laboratory confirmation along with donor verification becomes necessary.

Advantages of Urine Drug & Alcohol Screening

  • Recognized by DOT: It is the only DOT-approved and federally endorsed testing method.
  • Ideal for detecting recent use, making it key for workplace safety and post-incident reviews.
  • Offers expediency and cost-effectiveness.
  • Acceptance is widespread among courts, employers, and various government agencies.
  • Options for immediate tests are accessible for quicker screenings.
  • Showcases high accuracy, confirmed through GC/MS or LC/MS technologies.

Limitations of Urine Drug & Alcohol Screening

  • Detection spans modest durations (typically 1-7 days for most substances).
  • Potential risks for manipulation if security measures lack strength.
  • Lacks the capability to tell long-term substance histories.
  • Operates in environments needing specific restroom setups and collection methods.
  • Standard urine screens omit alcohol detection unless EtG/EtS analysis is engaged.

Despite certain constraints, urine testing prevails as the most viable and extensively implemented screening tool across the nation for drugs and alcohol.

How Hair Drug Testing Works

Middleburg, FL Hair Drug & Alcohol Testing Insights

Hair drug and alcohol testing stands out as an extremely reliable laboratory technique for assessing long-term substance use patterns. Unlike urine testing, which captures recent intake, hair testing covers a 90-day detection period by examining metabolites in the hair shaft, making it indispensable for workplace policies, court proceedings, child custody evaluations, and situations demanding extensive historical data.

Testing Procedures & Collection Specifics

Verification begins with a valid government ID, ensuring proper documentation through all necessary consent and custody signature forms.

A trained specialist collects a small segment usually 100-120 strands cut close to the scalp, typically from the crown. If head hair is unavailable, body hair serves as an alternative. The method is swift, pain-free, and observed directly.

A strict Hair Drug Test Custody and Control Form tracks the specimen through collection, sealing, and transfer, maintaining the legal soundness of data.

In the lab, hair samples undergo rigorous forensic processes:

Samples receive meticulous analysis:

Metabolites form part of the hair shaft, offering a durable record of substance intake. Given the internal accumulation, hair tests are challenging to falsify.

A certified Medical Review Officer evaluates all non-negative results to ensure accuracy, contemplates viable medical justifications, and releases the official result.

Timing for Hair Test Results in Middleburg, FL:

With extensive multi-step procedures, hair testing requires more time than urine evaluations but offers unparalleled consistency and accuracy.

Advantages of Hair Drug & Alcohol Testing

Challenges of Hair Drug & Alcohol Testing

Despite these drawbacks, hair testing prevails as a dependable approach for discerning long-term substance use across varied applications, revered by Middleburg, FL employers, legal representatives, and individuals for its resistance to tampering.

Alcohol & Drug Testing-How Each Method Works

Accredited Drug Testing extends a comprehensive assortment of drug and alcohol screening services, crafted to fulfill testing demands for employers, DOT, legal systems, and personal needs, even statewide in Middleburg, FL. Knowing how each testing method operates allows for accurate selection and dependable results, fully aligned with federal and state standards. Our broad network, certified labs, and expert Medical Review Officers uphold the necessary precision, trust, and professionalism for sound testing outcomes.

Alcohol Urine Drug Test

The alcohol urine test detects recent alcohol intake by finding ethanol metabolites within urine, mostly utilized in workplace testing, court cases, and post-accident scenarios. Though not measuring current impairment, it provides a brief detection window over several hours, presenting a swift, cost-effective choice for screening.

ETG Alcohol Test (Urine)

An EtG (Ethyl Glucuronide) urine test is a sensitive method discerning alcohol use up to 80 hours post-consumption. As EtG is a direct derivative of ethanol, this test is widely exercised by courts, rehab programs, and compliance monitoring organizations. Delivering a more extended timeline than standard alcohol urine tests, it's renowned for accuracy and consistency.

Breath Alcohol Test

A breath alcohol test gauges current impairment by analyzing the alcohol concentration in deep lung air. Utilizing DOT-sanctioned breath Alcohol Devices (EBTs), these tests provide instant, admissible results. Essential for DOT-covered employees, they're also prevalent in workplace, accident aftermath, and reasonable suspicion testing scenarios.

Blood Alcohol Test

The blood alcohol test directly quantifies ethanol in the bloodstream, esteemed for its accuracy in determining impairment. This method is utilized by hospitals, law officials, and legal entities, particularly valuable for DUI cases, emergency care, and intensive legal investigations.

PEth Alcohol Blood Test

A PEth (Phosphatidylethanol) blood test discerns chronic or substantive alcohol consumption by identifying unique alcohol biomarkers in red blood cells, sensitive up to 2-3 weeks. Regarded as among the most reliable indicators of extended drinking behaviors, it sees common use in family law, control programs, treatment centers, and sensitive industry pre-employment screenings.

ETG Alcohol Test (Hair)

Offering a 90-day detection range, the EtG hair alcohol test examines ethanol metabolites in the hair shaft, understanding repeated or chronic use rather than one-time events. Highly utilized in court hearings, child custody matters, and prolonged monitoring projects, the method is prized for stability, precision, and resistance to brief abstinence attempts.

With the competence of skilled experts, acclaimed national labs, and expedient scheduling, Accredited Drug Testing guarantees each alcohol testing method furnishes clear, defensible insights that assist individuals and businesses to stay knowledgeable, assured, and wholly compliant with Middleburg, FL's stringent regulations.


Accredited Drug Testing provides nationwide drug and alcohol screening solutions, including urine, hair, EtG, PEth, and breath alcohol testing. Backed by SAMHSA-certified laboratories, DOT 49 CFR Part 40 compliance, and scientifically validated testing technology, our services ensure accurate, defensible, and confidential results for employers, courts, and individuals.

Frequently Asked Questions

Urine tests detect recent use and are the only option approved for DOT testing under 49 CFR Part 40. Hair tests provide a 90-day detection window per the Society of Hair Testing (SoHT). Alcohol testing options, EtG, breath, blood, or PEth. These tests can identify recent use, impairment, or long-term drinking behavior depending on the method.

Urine: 24 - 48 hrs for negatives (SAMHSA guidelines).
Hair: 2 - 5 business days due to multi-step forensic processing (SoHT).
Breath Alcohol: Immediate results using NHTSA-approved EBT devices.
PEth: 3 - 5 days based on forensic toxicology studies.

Lab confirmation using GC/MS or LC/MS is the gold standard per SAMHSA & DOT.
Hair testing is highly tamper-resistant (SoHT).
PEth is considered the most reliable marker of repeated drinking (Journal of Analytical Toxicology).

No. DOT-regulated urine tests require validity checks and chain-of-custody documentation (49 CFR Part 40). Hair tests undergo decontamination and metabolite extraction (SoHT), making them extremely difficult to adulterate.

EtG urine detects alcohol for up to 80 hours (NIH/SAMHSA studies). EtG hair detects chronic alcohol consumption for up to 90 days (SoHT alcohol guidelines).

5 Panel DOT:49 CFR Part 40. Expanded non-DOT panels add benzodiazepines, barbiturates, methadone, oxycodone, MDMA, fentanyl, and more (SAMHSA forensic panel standards).

PEth blood testing identifies long-term and heavy alcohol use for 2 - 4 weeks. Used in child custody, treatment monitoring, and professional compliance programs (CDC & Journal of Analytical Toxicology).

Hair testing does not detect drug use within the past 5 - 7 days. It identifies long-term or repetitive use patterns per SoHT standards.

Yes. Results follow strict chain-of-custody procedures, DOT/SAMHSA requirements, GC/MS or LC/MS confirmation, and MRO review; making them acceptable in court, DOT, and legal settings.

Yes. Accredited Drug Testing offers same-day appointments at 20,000+ locations nationwide, instant testing options, and onsite employer testing with bulk discounts.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

Polite

I ran into a situation because the Guthrie had closed and I had to go to well care and they were right on it. I had no problems when I called to get connected with welcare everything worked out and I didn't even have to wait.they were polite and talked with the receptionist for me once I got them on the phone.

Sayre - 8/19/2024

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

ADT was able to get me the best tests

Dealing with allegation’s of drug use that completely false is stressful, ADT was able to get me the best tests, going back the farthest in the same day with as little hassle as possible, I spoke with 1 person and wasn’t transferred once, and they took my payment, and got me my work order for a clinic as close as possible for the service I needed. 5*

Christopher Hansis - 12/19/2024

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025


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(800) 221-4291