DOT Drug Testing - Middleburg, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 36 testing centers in the Middleburg area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

36 DOT Drug-Testing Centers in Middleburg

Minutes Away, Test Today

1708 Blanding Blvd

3.14 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1708 Blanding Blvd
Middleburg, FL 32068

Onsite Only

9.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Fleming Island, FL 32003

1680 Eagle Harbor Parkway, Suite B

9.66 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
1680 Eagle Harbor Parkway, Suite B
Orange Park, FL 32003

2140 Kingsley Ave

9.67 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2140 Kingsley Ave
Orange Park, FL 32073

2020 Kingsley Ave Ste A

9.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2020 Kingsley Ave Ste A
Orange Park, FL 32073

1895 Kingsley Ave Ste 803

9.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1895 Kingsley Ave Ste 803
Orange Park, FL 32073

1563 Kingsley Ave Ste 102

10.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1563 Kingsley Ave Ste 102
Orange Park, FL 32073

1465 Kingsley Ave Ste 1401,

10.48 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
1465 Kingsley Ave Ste 1401,
Orange Park, FL 32073

3185 Highway 17

11.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3185 Highway 17
Green Cove Springs, FL 32043

705 Ferris Street

11.95 mi
🕑 Testing Hours: M-Th 8:00 AM-5:00 PM • F 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
705 Ferris Street
Green Cove Springs, FL 32043

12303 San Jose Blvd

14.65 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
12303 San Jose Blvd
Jacksonville, FL 32223

5501 Roosevelt Blvd

15.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

7963 Normandy Blvd

16.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7963 Normandy Blvd
Jacksonville, FL 32221

6671 Hyde Grove Ave

16.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

1584 Normandy Village Parkway Ste 32

16.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

4616 San Juan Ave

16.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

1912 Hamilton St Ste 103

16.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

9826 San Jose Blvd

16.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9826 San Jose Blvd
Jacksonville, FL 32257

9857 Old Saint Augustine Rd Ste 4

17.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9857 Old Saint Augustine Rd Ste 4
Jacksonville, FL 32257

1610 Blanding Blvd

17.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

345 W Madison St

17.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

5964 Normandy Blvd

17.73 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

231 Airline Way

18.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

4221 Baymeadows Rd Ste 6

18.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4221 Baymeadows Rd Ste 6
Jacksonville, FL 32217

1550 S Water St

18.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

4498 Hendricks Ave

18.96 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

7033 Commonwealth Ave Ste 8

19.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

9143 Philips Hwy Ste 495

19.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9143 Philips Hwy Ste 495
Jacksonville, FL 32256

2063 Oak St

19.90 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2063 Oak St
Jacksonville, FL 32204

837 Edgewood Ave N

19.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
837 Edgewood Ave N
Jacksonville, FL 32254

7933 Baymeadows Way Ste 3

20.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

4933-1 University Blvd W

20.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4933-1 University Blvd W
Jacksonville, FL 32216

4237 Salisbury Rd Ste 304

20.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

6639 Southpoint Pkwy Ste 106

20.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

4063 Salisbury Rd Ste 205

20.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

1325 San Marco Blvd Ste 301

20.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 36 DOT drug testing locations to serve you in and around Middleburg, FL.

In Middleburg, FL, Accredited Drug Testing centers cater to local businesses and operators offering DOT Drug and Alcohol Testing, DOT Physicals, and other required services to comply with 49 CFR Part 40, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA requirements. With convenient locations near your home or office, same-day service is frequently available.

Select your mandated DOT service or a drug/alcohol test at a nearby center. Testing is streamlined and user-friendly for individuals, employees, or others, simplifying arrangement of DOT drug testing in Middleburg.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Middleburg, Florida DOT Services

DOT Drug Testing & Services Available in Middleburg, FL:

In Middleburg, FL, Accredited Drug Testing offers comprehensive DOT services including drug and alcohol testing. Convenient locations and flexible scheduling cater to your busy lifestyle.

DOT Services in Middleburg, FL

DOT regulations ensure drug and alcohol testing maintains safety for employees and the public. In Middleburg, Florida, Accredited Drug Testing assures compliance with rigorous DOT guidelines.

Testing services in Middleburg, FL, include pre-employment, random, reasonable suspicion, return-to-duty, and follow-up tests for all safety-sensitive employees.

Strict procedures are in place to prevent workplace incidents, safeguarding both employees and operations in Middleburg, FL, by adhering to DOT standards.

Maintaining safety and integrity, DOT-compliant testing in Middleburg, Florida, builds a reliable workforce, reducing risk through accountable and transparent procedures.

FMCSA Information

In Middleburg, FL, FMCSA regulations require testing for truck drivers to maintain safety and compliance through routine checks and thorough documentation.

Middleburg businesses must ensure FMCSA compliance by enrolling in drug and alcohol programs, integral for safe transportation operations.

USCG Information

USCG rules in Middleburg, FL, enforce stringent testing for maritime personnel, critical for maritime security and compliance.

Middleburg's maritime operations adhere to USCG regulations, ensuring robust safety measures on water.

FAA Information

FAA regulations in Middleburg, FL enforce drug and alcohol testing for aviation professionals, crucial for maintaining air travel safety and compliance.

Middleburg's FAA regulations require comprehensive testing programs, essential for flight operation integrity and safety.

FRA Information

In Middleburg, FL, FRA rules mandate rigorous drug testing for rail employees, essential for secure and efficient railway operations.

Middleburg businesses follow FRA guidelines to ensure employee sobriety, paramount for railway safety and reliability.

FTA Information

FTA regulations in Middleburg, FL, emphasize testing for public transportation workers, maintaining transit safety through routine checks.

Public transport in Middleburg incorporates FTA-compliant testing for a secure travel environment.

PHMSA Information

PHMSA regulations in Middleburg, FL, focus on workers handling hazardous materials, mandating regular testing to uphold safety standards.

Middleburg operations comply with PHMSA by adhering to drug and alcohol testing protocols, critical for logistical safety.

Scheduling in Middleburg, FL

To book any DOT drug or alcohol test in Middleburg, FL, contact us at (800) 221-4291 or utilize our online system by choosing your test and filling out the necessary registration information prior to your visit.

The entered zip code identifies the closest testing center in Middleburg, FL offering your selected test. We email you a registration form including the address, hours, and instructions, which you should present at the center.

Our facilities hold SAMHSA certification, with all results verified by in-house Medical Review Officers (MROs).

Besides offering DOT testing in Middleburg, FL, Accredited Drug Testing has numerous facilities throughout the area and beyond.

Traveling? Relax, as we provide easy scheduling for tests nationwide, available anywhere, anytime!

Florida Dept. of Transportation Updates

DUI Statistics in Clay County, FL

Driving Under the Influence (DUI) remains a concerning issue in Clay County, Florida. While specific detailed statistics for Middleburg, FL, may not be readily accessible, data from the County level provides a representative understanding. In recent years, the DUI-related incidents have shown various trends, highlighting the significance of targeted interventions. The county, known for its commuter population and proximity to larger cities, faces challenges due to the high volume of traffic and diverse demographic. The Florida Department of Transportation (FDOT), alongside local authorities, has actively implemented measures to curb DUI incidents through increased checkpoints, educational campaigns, and community involvement. These efforts aim to reduce not just occurrences but also recidivism among offenders, focusing on comprehensive rehabilitation and awareness initiatives.

Drug-Involved Accidents in Clay County, FL

Clay County, Florida, like many regions, battles the complex issue of drug-related vehicular incidents. Although specific statistics for Middleburg, FL, might be limited, Clay County serves as an indicative zone for analysis. Drug-involved accidents encompass a range of substances, from prescription drugs to illicit narcotics. The rising trend of accidents where drugs were a factor has prompted local government and the Florida Department of Transportation (FDOT) to amplify efforts in combating this issue. Strategies include enhancing law enforcement training, public education on the dangers of driving under the influence of drugs, and fostering community-based programs for substance abuse treatment. Additionally, statistical analysis and research are ongoing to better understand the root causes and develop more effective prevention strategies tailored to the needs of Clay County and its towns, including Middleburg.

Marijuana-Related Accidents in Clay County, FL

The legal discourse surrounding marijuana has broadened nationwide, influencing driving behaviors and accident statistics in regions like Clay County, Florida. While Middleburg, FL, specific data may not be disaggregated, county-level information shows an emerging pattern of marijuana-related driving incidents. As marijuana laws evolve, with medical use legally recognized in Florida, there is cautious monitoring of its impact on road safety. The Florida Department of Transportation (FDOT) and local authorities in Clay County emphasize rigorous data collection and analysis to understand marijuana's role in vehicular accidents. Public awareness campaigns targeting marijuana users have been ramped up to address and mitigate the risk of impaired driving. Moreover, there is ongoing collaboration with health professionals to educate citizens on the potential dangers of operating vehicles under the influence of cannabis, focusing on creating a safer environment for all road users.

Middleburg Drug Testing

Quickly find trusted local drug testing centers in Middleburg, FL — fast, convenient, and reliable every time!

Middleburg DOT Drug Testing

Quickly find a local DOT drug testing center in Middleburg, FL — fast, reliable, convenient nationwide service!

Middleburg DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middleburg, FL.

Middleburg Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middleburg, FL.v

Middleburg Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middleburg, FL locations—results you can trust, every time!

Middleburg Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middleburg, FL.

Middleburg Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middleburg, FL.

Middleburg 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middleburg, FL employers and individuals nationwide.

Middleburg 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middleburg, FL workplace and personal testing needs.

Middleburg On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middleburg, FL workplace compliant.

Middleburg DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middleburg, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Fast and easy

It was fast and easy a pleasure to talk to the customer service people great service

Peter Villegas - 1/19/2025

Unbelievably fast and Easy

Unbelievably fast and Easy, I wont be using Any other provider from now on.

Ren Wisby - 4/19/2025

Fantastic Experience

Fantastic experience from the beginning. Jocelyn was very helpful to me as someone who doesn’t have much experience in needing a test. They were very helpful and walked me through the entire process start to finish. Thank you ADT!

Riley Wilson - 2/18/2025

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025

Very helpful

They were very helpful in finding a place to get tested on the route back home! They made a difficult time seem easy with no judgement!

Anne Creden - 11/19/2024


(800) 221-4291