DOT Drug Testing - Chicago, IL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 33 testing centers in the Chicago area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

33 DOT Drug-Testing Centers in Chicago

Minutes Away, Test Today

2361 Reliable Parkway

0.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2361 Reliable Parkway
Chicago, IL 60686

2393 Reliable Parkway

0.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2393 Reliable Parkway
Chicago, IL 60686

3145 S Ashland Ave Ste 110

1.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3145 S Ashland Ave Ste 110
Chicago, IL 60608

2907 S Wabash Ave Ste 100

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2907 S Wabash Ave Ste 100
Chicago, IL 60616

3201 S Wallace St

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3201 S Wallace St
Chicago, IL 60616

3316 S Ashland Ave

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3316 S Ashland Ave
Chicago, IL 60608

2600 S Michigan Ave

1.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2600 S Michigan Ave
Chicago, IL 60616

811 S State St Ste B

1.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
811 S State St Ste B
Chicago, IL 60605

2929 S Ellis Ave

1.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2929 S Ellis Ave
Chicago, IL 60616

600 W Adams St

2.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
600 W Adams St
Chicago, IL 60661

75 Remittance Dr Ste 1660

2.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
75 Remittance Dr Ste 1660
Chicago, IL 60675

Remit Only: Dept 77-6478

2.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Remit Only: Dept 77-6478
Chicago, IL 60678

614 W Monroe St

2.14 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
614 W Monroe St
Chicago, IL 60661

300 W Adams St Ste 835

2.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 W Adams St Ste 835
Chicago, IL 60606

1230 W Lake St

2.49 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 7:30 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
1230 W Lake St
Chicago, IL 60607

36 W Randolph St Ste 705

2.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 W Randolph St Ste 705
Chicago, IL 60601

55 E Washington St Ste 2500

2.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
55 E Washington St Ste 2500
Chicago, IL 60602

311 N Aberdeen St Ste 100b

2.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 N Aberdeen St Ste 100b
Chicago, IL 60607

111 N Wabash Ave Ste 1514,

2.63 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
111 N Wabash Ave Ste 1514,
Chicago, IL 60602

300 E Randolph St Cl404

2.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 E Randolph St Cl404
Chicago, IL 60601

835 S Wolcott Ave Ste E144

2.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
835 S Wolcott Ave Ste E144
Chicago, IL 60612

676 N Saint Clair St Ste 900

3.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
676 N Saint Clair St Ste 900
Chicago, IL 60611

1030 W Chicago Ave

3.22 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM • Sat: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1030 W Chicago Ave
Chicago, IL 60642

219 W Chicago Ave

3.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
219 W Chicago Ave
Chicago, IL 60654

650 W 51st St

3.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
650 W 51st St
Chicago, IL 60609

233 E Erie St Ste 602

3.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
233 E Erie St Ste 602
Chicago, IL 60611

1460 N Halsted St Ste 201,

4.00 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM • Sat: 8:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
1460 N Halsted St Ste 201,
Chicago, IL 60642

5709 S. Loomis Blvd

4.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5709 S. Loomis Blvd
Chicago, IL 60636

1044 N Francisco Ave

4.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1044 N Francisco Ave
Chicago, IL 60622

1520 N Damen Ave

4.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1520 N Damen Ave
Chicago, IL 60622

1431 N Western Ave Ste 137,

4.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1431 N Western Ave Ste 137,
Chicago, IL 60622

5841 S Maryland Ave

4.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5841 S Maryland Ave
Chicago, IL 60637

2400 N. Ashland Ave Suite 100

5.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2400 N. Ashland Ave Suite 100
Chicago, IL 60614

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 33 DOT drug testing locations to serve you in and around Chicago, IL.

Our Chicago, IL-based DOT Drug Testing facilities cater to DOT Drug Testing, Alcohol Testing, Physicals, Random pool and Consortium enrollment, and numerous DOT Services ensuring compliance with 49 CFR Part 40. Available for Employers, Single operators across FMCSA, FTA, FAA, USCG, FRA, and PHMSA, centers offer convenient, often same-day, service near you.

Select your DOT service or drug/alcohol test(s) and opt for a nearby location in Chicago, IL—testing is accessible for yourself, employees, or others. With a seamless process, setting up your DOT drug testing near Chicago is hassle-free.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Chicago, Illinois DOT Services

DOT Drug Testing & Services Available in Chicago, IL:

In Chicago, IL, our DOT services streamline drug and alcohol testing processes, ensuring compliance with federal regulations, safety, and operational efficiency among transportation employees.

DOT Services in Chicago, IL

Understanding and complying with DOT regulations ensures safety and reliability among employees in all transportation sectors in Chicago, Illinois. Drug and alcohol screening safeguard public and worker safety.

DOT mandates strict screening protocols ensuring Chicago employers comply with federal regulations, minimizing accidents and fostering a drug-free, safe work environment.

In Chicago, Illinois, DOT screenings ensure only qualified, sober individuals operate commercial transport, significantly reducing risks on the roads, rails, skies, and seas.

Employee safety in Chicago is paramount, with DOT-regulated screenings providing essential measures to detect and deter substance abuse across various transportation industries.

FMCSA Information

In Chicago, IL, FMCSA regulates drug and alcohol screening for commercial driver's license holders, maintaining public and driver safety throughout the transportation network.

Chicago's FMCSA guidelines emphasize regular, randomized testing to prevent substance abuse among commercial drivers, ensuring responsible and safe operation of vehicles.

USCG Information

The USCG applies drug and alcohol testing regulations to Chicago, IL's maritime workforce, crucial for safety compliance on navigable waters.

In Chicago, USCG's strict screening curtails substance abuse, protecting lives and property within the maritime sectors south of the Great Lakes.

FAA Information

In Chicago, IL, FAA closely monitors drug and alcohol testing adherence in aviation to maintain high safety standards for airline staff and passengers alike.

FAA drug testing in Chicago is crucial for preserving the integrity and safety of air travel, deterring substance use among aviation personnel.

FRA Information

FRA in Chicago, IL, mandates extensive drug and alcohol testing for rail industry workers, safeguarding passengers and freight operations by minimizing avoidable risks.

Ensuring sober operation, FRA's regulations in Chicago uphold strict testing policies for rail staff, promoting safety and reliability on the tracks.

FTA Information

FTA enforces drug and alcohol testing rules in Chicago, IL, for transit systems, ensuring public confidence in safe, substance-free public transportation services.

Chicago's FTA regulations advocate seamless transit operations by maintaining stringent testing standards for public transport employees.

PHMSA Information

PHMSA enforces stringent drug and alcohol testing rules in Chicago, IL, ensuring safe transportation of hazardous materials across various sectors.

Chicago ensures compliance with PHMSA's protocols, achieving optimal security and safety in managing and transporting hazardous materials via regular screening.

Scheduling in Chicago, IL

Contact our account managers at (800) 221-4291 for scheduling any DOT drug or alcohol test in Chicago, IL. Use our online express registration by selecting a test and completing your details. Registration is mandatory before visiting the facility.

Enter your zip code to locate the nearest Chicago testing center for the selected DOT drug or alcohol test. Following registration, a donor pass with location details and instructions will be emailed. Walk-ins are welcome but ensure the donor section is complete and the test payment is made.

We utilize SAMHSA-certified labs, and our in-house licensed physicians, functioning as Medical Review Officers (MRO), securely verify all test results.

Accredited Drug Testing operates additional centers across Chicago, IL, and the nearby areas, ensuring easy access to DOT drug and alcohol testing services.

Available nationwide, Accredited Drug Testing offers unparalleled convenience with centers in numerous cities for scheduling tests anywhere, anytime, even during travels.

Illinois Dept. of Transportation Updates

DUI Statistics in Chicago, IL

Driving under the influence (DUI) remains a significant concern for the Department of Transportation (DOT) in Chicago, IL. The city sees numerous DUI-related incidents annually, posing serious risks to public safety. In Illinois, stringent DUI laws are enforced to deter impaired driving, with fines, license suspensions, and possible jail time for offenders. In recent years, the state's focus has been on reducing alcohol-impaired fatalities through increased law enforcement presence and public awareness campaigns. Cook County, where Chicago is situated, consistently records a high number of DUI arrests, highlighting the need for continued initiatives aimed at educating the public about the dangers of driving under the influence of alcohol or drugs.

Drug Involved Accidents in Chicago, IL

Drug involved accidents are a critical issue for the DOT in Chicago, IL. These incidents encompass crashes where drivers were found to have illegal drugs or prescription medications that impair driving abilities in their system. Illinois law enforcement agencies collaborate with the DOT to address this problem through roadside drug testing and enhanced patrol measures. Chicago, being an urban hub, experiences a substantial number of such accidents, and the authorities are investing in better detection and prevention strategies. Efforts include promoting public education on the dangers of drug-impaired driving and implementing advanced training programs for officers to efficiently recognize and manage drug-influenced drivers on the roadways.

Marijuana Related Accidents in Chicago, IL

The legalization of marijuana in Illinois, including Chicago, has brought forth challenges concerning marijuana-related accidents. The DOT in Chicago is actively engaged in addressing these concerns by studying the correlation between marijuana use and vehicular accidents. Post-legalization, there has been an increased focus on understanding THC's impact on driving capabilities and ensuring that measures are taken to prevent impaired driving. Law enforcement and the DOT promote campaigns aimed at educating the public about the dangers of driving under the influence of marijuana and are enhancing testing protocols to better identify intoxicated drivers. This approach aims to safeguard roads and reduce occurrences of marijuana-related incidents.

Chicago Drug Testing

Quickly find trusted local drug testing centers in Chicago, IL — fast, convenient, and reliable every time!

Chicago DOT Drug Testing

Quickly find a local DOT drug testing center in Chicago, IL — fast, reliable, convenient nationwide service!

Chicago DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Chicago, IL.

Chicago Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Chicago, IL.v

Chicago Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Chicago, IL locations—results you can trust, every time!

Chicago Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Chicago, IL.

Chicago Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Chicago, IL.

Chicago 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Chicago, IL employers and individuals nationwide.

Chicago 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Chicago, IL workplace and personal testing needs.

Chicago On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Chicago, IL workplace compliant.

Chicago DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Chicago, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

ADT was able to get me the best tests

Dealing with allegation’s of drug use that completely false is stressful, ADT was able to get me the best tests, going back the farthest in the same day with as little hassle as possible, I spoke with 1 person and wasn’t transferred once, and they took my payment, and got me my work order for a clinic as close as possible for the service I needed. 5*

Christopher Hansis - 12/19/2024

Polite

I ran into a situation because the Guthrie had closed and I had to go to well care and they were right on it. I had no problems when I called to get connected with welcare everything worked out and I didn't even have to wait.they were polite and talked with the receptionist for me once I got them on the phone.

Sayre - 8/19/2024

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025

Extremely pleasant

Initially Torrie helped me but I needed to call back. When I called back Kayla stepped in and finished my order. Both were extremely pleasant over the phone and provided wonderful customer service. Kudos to them!

Brynne Beverly - 4/19/2024

Really Helpful

I am just really happy to see that the location near me still offers hair follicle tests!! They are really helpful when any false positives happen, waiting on my results. Hope they come fast!

Kali Collins - 1/16/2025

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Kind and very helpful

Called around for hours and posted from company to person and got nowhere and had to fight to get a refund die to there miss information, well save time and sanity Acredited is the ONLY place the person was kind and very helpful, said what I needed and Faith the lady I dealt with made it simple and she did above and beyond everything I needed, as I'm going through a devorse this was a great help and made my day 100 stars from me.

Steven Harrison - 12/19/2024

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025


(800) 221-4291