DOT Physical Exams - Denver, CO

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With 34 testing centers in the Denver area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

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34 DOT Drug-Testing Centers in Denver

Minutes Away, Test Today

1670 Broadway 28th Fl

0.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1670 Broadway 28th Fl
Denver, CO 80202
Call (800) 221-4291 Get Donor Pass

777 Bannock St

0.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
777 Bannock St
Denver, CO 80204
Call (800) 221-4291 Get Donor Pass

1835 Franklin St

0.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1835 Franklin St
Denver, CO 80218
Call (800) 221-4291 Get Donor Pass

1730 Blake St Ste 100

1.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1730 Blake St Ste 100
Denver, CO 80202
Call (800) 221-4291 Get Donor Pass

1920 High St

1.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1920 High St
Denver, CO 80218
Call (800) 221-4291 Get Donor Pass

1515 Wazee Ste D

1.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1515 Wazee Ste D
Denver, CO 80202
Call (800) 221-4291 Get Donor Pass

2419 E 5th Ave

1.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2419 E 5th Ave
Denver, CO 80206
Call (800) 221-4291 Get Donor Pass

2490 W 26th Ave Ste 200a

2.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2490 W 26th Ave Ste 200a
Denver, CO 80211
Call (800) 221-4291 Get Donor Pass

120 Bryant St

2.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 Bryant St
Denver, CO 80219
Call (800) 221-4291 Get Donor Pass

3801 Brighton Blvd

2.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3801 Brighton Blvd
Denver, CO 80216
Call (800) 221-4291 Get Donor Pass

695 S Broadway,

2.42 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
695 S Broadway,
Denver, CO 80209
Call (800) 221-4291 Get Donor Pass

2420 W 26th Ave Ste200d

2.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2420 W 26th Ave Ste200d
Denver, CO 80211
Call (800) 221-4291 Get Donor Pass

1212 S Broadway Ste 150

3.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1212 S Broadway Ste 150
Denver, CO 80210
Call (800) 221-4291 Get Donor Pass

469 S Cherry St Ste 101

3.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
469 S Cherry St Ste 101
Denver, CO 80246
Call (800) 221-4291 Get Donor Pass

875 S Colorado Blvd

3.54 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
875 S Colorado Blvd
Denver, CO 80246
Call (800) 221-4291 Get Donor Pass

4101 E Louisiana Ave Ste 100

3.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4101 E Louisiana Ave Ste 100
Denver, CO 80246
Call (800) 221-4291 Get Donor Pass

Onsite Testing Only 3924 Wolff St

4.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Testing Only 3924 Wolff St
Denver, CO 80212
Call (800) 221-4291 Get Donor Pass

420 E 58th Ave Ste 111

4.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 E 58th Ave Ste 111
Denver, CO 80216
Call (800) 221-4291 Get Donor Pass

5855 E Stapleton Dr N Ste A130

4.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5855 E Stapleton Dr N Ste A130
Denver, CO 80216
Call (800) 221-4291 Get Donor Pass

1780 S Bellaire St Ste 302

4.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1780 S Bellaire St Ste 302
Denver, CO 80222
Call (800) 221-4291 Get Donor Pass

6750 E Stapleton Dr S

4.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6750 E Stapleton Dr S
Denver, CO 80216
Call (800) 221-4291 Get Donor Pass

6900 E 47th Avenue Dr Ste 100

4.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6900 E 47th Avenue Dr Ste 100
Denver, CO 80216
Call (800) 221-4291 Get Donor Pass

5720 Holly St

5.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5720 Holly St
Commerce City, CO 80022
Call (800) 221-4291 Get Donor Pass

5101 Quebec St

5.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5101 Quebec St
Commerce City, CO 80022
Call (800) 221-4291 Get Donor Pass

1864 S. Wadsworth Blvd #3

5.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1864 S. Wadsworth Blvd #3
Lakewood, CO 80232
Call (800) 221-4291 Get Donor Pass

5275 Marshall St Ste 201

5.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5275 Marshall St Ste 201
Arvada, CO 80002
Call (800) 221-4291 Get Donor Pass

499 E Hampden Ave Ste 290

5.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
499 E Hampden Ave Ste 290
Englewood, CO 80113
Call (800) 221-4291 Get Donor Pass

125 E Hampden Ave

5.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
125 E Hampden Ave
Englewood, CO 80113
Call (800) 221-4291 Get Donor Pass

7500 W Mississippi Ave Ste A 230

5.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7500 W Mississippi Ave Ste A 230
Lakewood, CO 80226
Call (800) 221-4291 Get Donor Pass

730 W Hampden Ave Ste 200

5.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
730 W Hampden Ave Ste 200
Englewood, CO 80110
Call (800) 221-4291 Get Donor Pass

901 W Hampden Ave Unit 103

5.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
901 W Hampden Ave Unit 103
Englewood, CO 80110
Call (800) 221-4291 Get Donor Pass

7585 W Arkansas Ave Ste B

6.09 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
7585 W Arkansas Ave Ste B
Lakewood, CO 80232
Call (800) 221-4291 Get Donor Pass

3601 S Clarkson St

6.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3601 S Clarkson St
Englewood, CO 80113
Call (800) 221-4291 Get Donor Pass

P O Box 179 Mail Stop 1398

6.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
P O Box 179 Mail Stop 1398
Littleton, CO 80201
Call (800) 221-4291 Get Donor Pass

Denver, CO DOT Physical - What You Need to Know

A DOT physical is an extensive health evaluation stipulated by the Department of Transportation (DOT) for all commercial vehicle drivers. This examination verifies that drivers are physically, mentally, and emotionally fit for safely navigating large vehicles on public roads. Conducted by a certified medical examiner on the National Registry of Certified Medical Examiners, successfully completing the exam earns a DOT medical card, vital for maintaining a valid CDL under FMCSA rules. For guidance on DOT agency-specific physicals, dial us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of medical history and medications
  • Vision test (corrective glasses allowed; minimum criteria must be met)
  • Hearing checkup (either forced whisper or audiogram)
  • Measuring blood pressure and pulse
  • General physical check (review of body systems)
  • Urine test (testing for specific gravity, protein, sugar)

Upon completing these tests successfully, a Medical Examiner's Certificate (MEC) could be valid for up to 24 months. Some cases may demand shorter certification timeframes or additional specialist approvals in Denver, MO.

Who needs a DOT Physical?

A valid DOT medical card is mandatory if you:

  • Operate a commercial driver's license (CDL) class A, B, or C for interstate travel
  • Drive a vehicle exceeding 10,001 lbs. GVWR across state lines
  • Transport over 8 paying passengers or more than 15 non-paying passengers
  • Carry hazardous materials needing placards under DOT guidelines
  • Work for DOT-regulated employers like FMCSA, FAA, FRA, FTA or USCG necessitating DOT medical certifications. Denver, NM drivers must comply.

DOT Physical Near Denver, CO

Northeast Jefferson, CO

Western Hills, CO

Sheridan, CO

Englewood, CO

Holly Hills, CO

Lakewood, CO

Commerce City, CO

Wheat Ridge, CO

North Washington, CO

Berkley, CO

Mountain View, CO

Edgewater, CO

Glendale, CO

Center, CO

Colorado City, CO

What to bring with you to a DOT Physical?

  • Government-issued ID
  • Eyewear and its prescription
  • Medication list with doses
  • CPAP machine data, if used
  • Specialist approvals (cardiac issues, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs, if monitored

Ensure these items are prepared before your Denver, MD DOT examination to prevent potential delays or issues in the process.

What happens if I fail a DOT Physical?

  • No Medical card awarded
  • Temporary suspension
  • Refer to your primary doctor for treatment
  • FMCSA offers exceptions for specific medical cases
  • Employer will be notified
  • Next steps involve addressing medical concerns and reattempting the exam. Ensure complete adherence to Denver, NM's operational standards for effective results.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Less than 20/40 vision in both eyes, inability to distinguish traffic light colors, or inability to hear a forced whisper from 5 feet away.
  • Blood Pressure & Heart Disease: Hypertension uncontrolled (≥180/110 mmHg), recent heart attack, uncontrolled angina, stroke, or an uncleared defibrillator.
  • Diabetes: Uncontrolled diabetes resulting in low blood sugar episodes frequently or complications like neuropathy or impaired vision that hinder safe driving.
  • Sleep Apnea & Respiratory Conditions: Untreated sleep apnea causing drowsiness during the day or severe lung conditions obstructing oxygenation.
  • Neurological Conditions: Epilepsy or seizure disorders (unless exempt), or conditions leading to unconsciousness, dizziness, or uncontrolled shaking.
  • Substance Misuse: Current illicit drug use, alcohol dependency, or abuse of prescription drugs impacting driving.
  • Mental & Cognitive Disorders: Serious uncontrolled psychiatric conditions or cognitive impairments affecting judgment or reaction time.

Some matters might cause a temporary disqualification until medical documentation is supplied (controlled blood pressure, diabetes management). Others, like untreated seizures or defibrillators, are seen as permanently disqualifying by FMCSA.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

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Certified DOT physicals with quick, reliable exams to keep Denver, CO drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

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(800) 221-4291