DOT Physical Exams - Alachua, FL

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With 30 testing centers in the Alachua area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

30 DOT Drug-Testing Centers in Alachua

Minutes Away, Test Today

720 Sw 2nd Ave Ste 160a

0.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
720 Sw 2nd Ave Ste 160a
Gainesville, FL 32601

825 Northwest 23rd Ave., Unit 2

1.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 Northwest 23rd Ave., Unit 2
Gainesville, FL 32609

4881 Nw 8th Ave Ste 2a

4.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4881 Nw 8th Ave Ste 2a
Gainesville, FL 32605

4343 W Newberry Rd Ste 10

4.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4343 W Newberry Rd Ste 10
Gainesville, FL 32607

3925 Nw 43rd St

4.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Nw 43rd St
Gainesville, FL 32606

812 Nw 57th St

4.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
812 Nw 57th St
Gainesville, FL 32605

6121 Nw 1st Pl

4.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6121 Nw 1st Pl
Gainesville, FL 32607

1131 Nw 64th Ter Ste A

5.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1131 Nw 64th Ter Ste A
Gainesville, FL 32605

3581 Sw Archer Rd Units 30 And 40

6.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3581 Sw Archer Rd Units 30 And 40
Gainesville, FL 32608

14819 Nw 140th St

13.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14819 Nw 140th St
Alachua, FL 32615

14417 Nw 152nd Ln

14.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14417 Nw 152nd Ln
Alachua, FL 32615

420 Ne Santa Fe Blvd

20.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 Ne Santa Fe Blvd
High Springs, FL 32643

1550 S Water St

22.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

345 W Madison St

23.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

850 E Main St Attn Lab

26.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054

128 Nw 137th Dr

30.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669

1113 Nw 23rd Ave

34.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1113 Nw 23rd Ave
Chiefland, FL 32626

1109 Sw 10th St

34.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1109 Sw 10th St
Ocala, FL 34471

3470 Nw 57th Trl

34.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3470 Nw 57th Trl
Bell, FL 32619

31 Sw 12th St

34.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31 Sw 12th St
Ocala, FL 34471

1834 Sw 1st Ave Ste 201

35.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1834 Sw 1st Ave Ste 201
Ocala, FL 34471

2415 Sw College Rd

35.18 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2415 Sw College Rd
Ocala, FL 34471

1015 Se 17th St Ste 200

35.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Se 17th St Ste 200
Ocala, FL 34471

2685 Sw 32nd Place,

35.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2685 Sw 32nd Place,
Ocala, FL 34471

2100 Se 17th St Ste 902

35.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2100 Se 17th St Ste 902
Ocala, FL 34471

8602 Sw State Road 200, 103rd Street Plaza, Suite J

36.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8602 Sw State Road 200, 103rd Street Plaza, Suite J
Ocala, FL 34481

6160 Sw Highway 200

37.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6160 Sw Highway 200
Ocala, FL 34476

205 Zeagler Dr, Suite 201

37.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
205 Zeagler Dr, Suite 201
Palatka, FL 32177

6500 Crill Ave

38.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6500 Crill Ave
Palatka, FL 32177

6101 Crill Ave

38.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6101 Crill Ave
Palatka, FL 32177

Alachua, FL DOT Physical - What You Need to Know

The DOT physical is an in-depth health screening mandated by the U.S. Department of Transportation for drivers of commercial motor vehicles. This test is essential to ensure that drivers are fit physically, mentally, and emotionally to handle large vehicles safely on the highways. A certified medical examiner (CME) from the National Registry of Certified Medical Examiners (NRCME) conducts the assessment. Successfully passing the exam results in obtaining a DOT medical certificate, also known as a Medical Examiners Certificate or DOT medical card, necessary for keeping a CDL license valid under FMCSA rules. If you are subject to other DOT regulations and need help understanding the specific exam required, you can contact us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Health history review and medication assessment
  • Vision screening (corrective lenses allowed; minimum standards apply)
  • Hearing assessment (forced whisper or audiometry)
  • Blood pressure and pulse rate
  • Physical examination (systems review)
  • Urinalysis (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months. Some conditions may require shorter certification or specialist documentation in Alachua, KS.

Who needs a DOT Physical?

You must hold a valid DOT medical certificate (card) if you:

  • Have a commercial driver's license (CDL) - class A, B, or C for interstate commerce
  • Drive a vehicle weighing over 10,001 lbs. GVWR in interstate commerce
  • Transport over 8 passengers (for hire) or over 15 passengers (not for hire)
  • Move hazardous materials needing placarding as per DOT regulations
  • Work for DOT-regulated employers like FMCSA, FAA, FRA, FTA, or USCG needing DOT medical qualification in Alachua, AR

What to bring with you to a DOT Physical?

  • Government-issued photo identification
  • Eyeglasses/contact lenses & prescription
  • Medication list & dosages
  • CPAP use records (if applicable)
  • Specialist medical clearances (cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure records if under monitoring. Alachua, NJ.

What happens if I fail a DOT Physical?

  • Denial of Medical card issuance
  • Imposition of short-term disqualification
  • Recommendations for primary care consultation
  • FMCSA can provide certain medical conditional exemptions
  • Your employer receives notice regarding status
  • It is essential to voice the medical challenges, seek remedies, and take the exam again, especially within Alachua, OK.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision poorer than 20/40 in each eye even with correction, inability to differentiate traffic light colors, or hearing deficiency preventing detection of a forced whisper from 5 feet away.
  • Blood Pressure & Heart Disease: Unmanaged hypertension (≥180/110 mmHg), recent myocardial infarction or stroke, uncontrolled angina, or having an unapproved defibrillator.
  • Diabetes: Uncontrolled diabetes with recurrent hypoglycemia or issues like neuropathy that hinder safe driving.
  • Sleep Apnea & Respiratory Issues: Untreated sleep apnea causing daytime drowsiness, or serious lung diseases affecting breathing.
  • Neurological Disorders: Epilepsy (unless certified), conditions causing fainting or tremors.
  • Substance Abuse: Ongoing illegal drug use, alcohol dependence, or misuse of prescriptions affecting driving ability.
  • Psychiatric & Cognitive Disorders: Severe uncontrollable mental illnesses or cognitive disorders impacting judgment and response time.

Conditions can temporarily disqualify until medical clearance (e.g., regulated blood pressure), whereas others, like untreated seizures, may be permanent disqualifications under FMCSA rules.

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Alachua DNA Testing

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Alachua Hair Drug Testing

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Alachua Alcohol Testing

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Alachua Drug Testing Services

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Alachua 5 Panel Drug Test

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Alachua DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Alachua, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291