Urine| Hair Follicle | Oral Fluid | Blood | ETG
Hair analysis is recognized as a highly effective method for identifying drug and alcohol usage. The fibers of hair strands capture biomarkers, offering a long-term record of substance use. When sampled near the scalp, hair can reflect drug and alcohol consumption over a period of up to three months. Hair is straightforward to collect, challenging to tamper with, and convenient to transport.
A sample measuring 1.5 inches with about 200 hair strands, equivalent to a #2 pencil’s thickness, amounts to 100mg of hair, which is optimal for both screening and confirmation purposes. When testing for EtG, additional markers, or tests exceeding 10 panels, it is recommended to use 150mg of the specimen. Measuring the specimen on a jeweler's scale is advisable. In cases where scalp hair is not available, an equivalent amount of body hair can be used. References to head hair pertain strictly to scalp hair, while body hair encompasses all other hair types, including facial and axillary hair.
Process Overview
The laboratory drug testing process encompasses four primary stages: Accessioning, Screening, Extraction, and Confirmation.
During Accessioning, the sample undergoes initial processing into the laboratory's system. This process involves checking that the sample was correctly sealed and shipped, assigning a unique LAN (Laboratory Accessioning Number), and completing any necessary data entry absent from an electronic chain of custody system.
Screening comprises an initial rapid assessment for drugs of abuse. Although it is an economical approach to dismiss drug presence in most samples, any positive screening result requires confirmation to be court-admissible. Samples with a presumptive positive from Screening mandate additional confirmation.
For samples showing a presumptive positive at the Screening stage, additional hair is extracted from the initial specimen in preparation for Extraction. This stage involves extracting drugs from hair at notably lower concentrations than other testing methodologies (e.g., urine or oral fluid), underscoring the complexity of hair drug screening.
Positive screening confirmations are executed through GC/MS, GC/MS/MS, or LC/MS/MS. Any samples showing presumptive positives are washed before confirmation, as required. The comprehensive laboratory procedure from Accessioning to Confirmation undergoes review under both the CAP (College of American Pathologists) Hair classification and ISO / IEC 17025 standards accreditation.
Advantages of hair drug testing:
Limitations:
Note: Despite the common term "hair follicle tests", the analysis is conducted on the hair strand and not the follicle beneath the scalp.
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